Eduard married Margrete Holm in 1845 in Haderslev, Denmark. Margrete (daughter of Johan Kristian Holm and Bartoline Frederikke Schroeter) was born on 09 Mar 1823 in Haderslev, Denmark; died on 13 May 1906. [Group Sheet] [Family Chart]

1. Kristine Mathilde Frederikke Nissen was born on 05 Sep 1845 in Haderslev, Denmark.
2. Johan Karl Edvard Nissen was born on 23 Oct 1848 in Haderslev, Denmark; died on 18 May 1851 in Haderslev, Denmark.
3. Adolf Herman Nissen was born on 20 Dec 1849 in Haderslev, Denmark.
4. Margrete Katrine Helene Nissen was born on 09 Dec 1851 in Haderslev, Denmark; died on 29 May 1870 in Altona, Germany.
5. Bartoline Sofie Nissen was born on 05 Jan 1855 in Hohn, Germany.
6. Johan Karl Edvard Nissen was born on 21 Sep 1856 in Hohn, Germany; died on 26 Jan 1868 in Altona, Germany.
7. Anna Kristine Nissen was born on 13 Feb 1858 in Hohn, Germany.
8. Karl Georg Nissen was born on 25 Jul 1861 in Hohn, Germany.